Inhibition of microRNA-122 alleviates pyroptosis by targeting dual-specificity phosphatase 4 in myocardial ischemia/reperfusion injury

Pyroptosis is a type of programmed cell death that induces myocardial ischemia-reperfusion injury (I/RI), which leads to cardiac dysfunction and even lethal reperfusion injury. MiR-122 liver-specific miRNA associated with coronary heart disease, but its role in pyroptosis activation I/RI remains unclear. Thus, this study aimed determine whether miR-122 inhibition exerts protection vivo vitro models. An model was established using C57BL/J6 male mice. expression upregulated the tissues from group. Quantitative results echocardiography parameters showed improved function downregulated interleukin (IL)-1β, IL-18, caspase 1, 11. However, pretransfection recombinant adeno-associated virus 9 encoding DUSP4-specific siRNA (AAV9-siDUSP4) blocked protective effects inhibition. A hypoxia/reoxygenation (H/R) mimic I/R condition H9C2 cells. Results increased superoxide dismutase activity (SOD) viability decreased malondialdehyde (MDA) level, IL-1β, 11, death. These were abolished by transfection siRNA. In summary, I/RI, alleviates suppressing through targeting DUSP4. may be novel therapeutic target for treating I/RI.